056
(
\. L
PAGGI,MARTIN & DEL BENE ILP
Consulting Engineers & Land Surveyors
Drj0
56 Main Street
Poughkeepsie, New York 12601
845-471-7898
845-471-0905 (FAX)
November 6, 2000
Town Board
Town of Wappinger
P.O. Box 324
Wappingers Falls, New York 12590
RECEIIIEL
NOV C' n
" 2000
~~/1tE
row", ~DEJ;lf
Attention: Constance O. Smith, Supervisor
Reference: Tri-Municipal Sewer Commission Meeting
Dear Supervisor Smith:
I attended the November 2nd, 2000 Commission meeting, and enclose the
correspondence handed out at the meeting. Of importance at the meeting were
the following:
1. They are projecting to put their Contracts out to bid the first week in
November 2000 and open mid-December.
2. The Department of State must act on the easement for the creek crossing
for Wappinger Creek.
3. The Environmental Facilities Corporation (EFC) Contract adopted by the
Town of Wappinger was adopted by the Commission that evening and will
be forwarded up to EFC.
4. A presentation was made by Malcolm Pirnie on the air samplings at the
treatment plant.
If there are any questions on the above, please do not hesitate to contact this
office.
ve'J}:fIY. yours, ~
Y~<'J
~ Joseph E: Paggi, Jr., P. .
JEP:law
Enclosure
cc: Hon. Vincent Bettina, Councilman w/enclosure
Hon. Joseph Paoloni, Councilman w/enclosure
Hon. Joseph Ruggiero, Councilman w/enclosure
Hon. Robert Valdati, Councilman w/enclosure
Hon. Elaine Snowden w/enclosure
Joseph E. Paggi, Jr., P.E.
Ernst Martin, Jr., P.E., L.S.
Charles R. Del Bene, Jr., P.E.
~ ,d'2-1oo
~
MDRA
MATTHEW D. RUDIKOFF ASSOCIATES, INC.
Riverside at Beacon
7-11 Beekman Street · P.O. Box 271 · Beacon, New York 12508
TEL (914) 831-1182. FAX (914) 831-2696
MEMORANDUM
TO:
Robert Juliano, Chairman and Commissioners
FROM:
Matthew D. Rudikoff, President
DATE:
November 2,2000
RE:
Permit Status Update - Wappinger Phase 3A
Our File No. - TM50572
I spoke with Mike Merriman New York State Department of Environmental Conservation
(NYSDEC) concerning the status of the NYSDEC's permit reviews for the 3A expansion.
JRegarding the SPDES Permit Modification:
The Draft Permit has been issued by the DOC Waters Section. The procedure at this point is that
DEC send the draft permit to the TMSC for the TMSC to review and place a public hearing
announcement in the newspaper. This initiates a thirty day comment period. Ifno substantive and
significant comments are received from the public DEC can issue the SPDES permit.
J Regarding the Army Corps for the Wappinger Creek crossing:
Responses have been made to the plans based upon the review letter from the Army Corps of
Engineers (ACOE). Those changes when completed must be submitted to the ACOE to complete
the application. The ACOE then has up to 45 days for their determination that the Nationwide
Permit (NWP - 12 Utility Line Activities) is applicable. Dr. Bridges of my staffhas been in touch
with Brian Orzel of the ACOE who has reiterated the opinion that he believes the project will be
able to proceed under NWP -12.
\,) Regarding the DEC Water Quality Individual Certification:
Mike Merriman informed me that DEC has determined that it has no jurisdiction over the
Wappinger Creek or other 3 intermittent creek crossings or other aspects of the proposed
activities and therefore will opt to not require a Part 404 Individual Water Quality Assurance
Certification.
Tri-Municipal Sewer Commission / Permit UpdatelPhase 3A Plant Expansion / TM50572/ November 2, 2000
Page 1 of 2
MATTHEW D. RUDIKOFF ASSOCIATES, INC.
Regarding Solid Waste:
Mike Merriman has confirmed that the rates of solids generation after the expansion will be
below our current Part 360 Permit limit of2.9 dry tons per day based upon our submission to
him. He informed me that he believed that the Solid Waste Division would not feel a permit
modification was required. Mike has not heard back from them and is confirming their earlier
OpInIOn.
MDRA will continue to monitor the final stages of these procedures.
cc: Tri-Municipal Sewer Commission
Anthony Quartararo
lnder Kumar
Constance Smith
Jay Paggi
Al Roberts
Tri-Municipal Sewer Commission / Permit UpdatelPhase 3A Plant Expansion / TM50572/ November 2, 2000
Page 2 of 2
":::",-- ~
TRI-MUNICIPAL SEWER COMMISSION
TREASURER'S REPORT
OCTOBER 1,2000 THROUGH OCTOBER 31, 2000
OPERATION & MAINTENANCE
CASH IN BANK- OCTOBER 1, 2000
HSBC
089-66119-6 Savings Account
089-20418-6 Checking Account
089-23452-2 Payroll Account
RECEIPTS
Severn Trent Environmental (phone)
Village of Wappinger Falls
Town of Wappinger
Town of Poughkeepsie
3-A October
TOTAL
DISBURSEMENTS
See attached schedule
CASH IN BANK-
OCTOBER 31, 2000
LOCA TION OF FUNDS
HSBC
089-66119-6 Savings Account
089-20418-6 Checking Account
089-52378-8 CD
089-23452-2 Payroll Account
Respectfully submitted,
Ann Barcher
Treasurer
h~ll t~too
$314,321.42
5,504.29
562.66 $320,388.37
22.06
105,125.00
89,087.00
27,551.00
1,000.00 222,785.06
543,173.43
57,655.07
$485,518.36
$228,154.74
$6,806.10
250,000.00
557.52 $485,518.36
10/20/00 11:38 am
Chk. #
7489
7490
7491
7492
7493
7494
7495
7496
7497
7498
7499
7500
7501
7502
7503
7504
7505
7506
7507
7508
7509
7510
7511
7512
7513
7514
7515
7516
7517
7518
7519
7520
7521
7522
7523
7524
7525
7526
Date
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
la/aS/aD
10/05/00
la/aS/aD
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
10/05/00
la/aS/DO
10/20/00
Payroll :
22 10/11/00
23 10/25/00
Tri-Municipal Sewer Commission
P.O. Box 859.
Wappingers Falls, NY
Marine Midland Checking
Printed Checks - Regular.
For October 2000
Page
1
Payee Name
Total Amount
88.18
62.04
261.62
65.54
68.51
418.64
63.59
33.97
6,,966.09
786.77
122.00
68.25
284.25
1,150.00
46.54
30.00
567.23
1,505.00
1,312.97
460.46
34.87
3,060.05
277.68
83.22
82.68
8.27
375.00
204.20
93.69
22.51
23.25
300.33
1,274.03
32,181. 00
446.46
239.80
440.00
3,193.90
A T & T
AWESCO
Verizon
Verizon
Verizon
Bottini Fuel
Cash
Cellular One
Central Hudson
Central Hudson
CUSTOM MONITORING
Jacqueline Donnellan
ELECTRO-CONTROL SUPPLY, INC.
Ennix Inc.
GALLAGHER TRUCK CENTER INC.
Home Depot/GECF
H. O. Penn Machinery Co., Inc.
H. O. Penn Machinery Co., Inc.
Jones Chemicals Inc
Lowes
Lucent Technologies
LOUIS MILLER PLUMBING, HTG & CONSULT
HSBC
NYS EMPLOYMENT TAXES
NYS INDUSTRIES FOR THE DISABLED, INC
Office Max
Quartararo & Quartararo
Royal Carting
R. S. MEANS COMPANY, INC.
Sarjo Industries
Sears Commercial Credit Central
Sofco, Inc
Sedore, O'Sullivan,Letterio & Barsch
Severn Trent Environmental Services
U S MATERIALS HANDLING CORP
W. W. Grainger Inc.
R.C. Zimmermann
Central Hudson
J. Donnellan
J. Donnellan
478.80
473.68
$57 ;. 655.07
Total Disbursements:
\
TRI-MUNICIPAL SEWER COMMISSION
TREASURERS REPORT
OCTOBER 1,2000 THROUGH OCTOBER 31,2000
TOWN OF WAPPINGER EXPANSION
CASH IN BANK- OCTOBER 1, 2000
HSBC
089-65269-3 Savings Acct (Final Design)
089-20980-3 Checking Account
RECEIPTS
Town of Wappinger
Town of Wappinger
TOTAL
DISBURSEMENTS
Phase 3A
Quartararo & Quartararo
Quartararo & Quartararo
Quartararo & Quartararo
M D Rudikoff Assoc.
Sedore & Co.
Tri-Muni 0 & M
Eustance & Horowitz
M D Rudikoff Assoc.
Ethan P. Jackman
CHECK#
$923.91
100.00 1,023.91
26,540.58
3,000.00 29,540.58
30,564.49
AMOUNT
2354
2355
2356
2357
2358
2359
2360
2361
2362
Total Phase 3A
CASH IN BANK-
OCTOBER 31,2000
LOCATION OF FUNDS
HSBC
089-65269-3 Savings Acct (Final Design)
089-20980-3 Checking
Respectfully Submitted,
Ann Barcher
Treasurer
$5,556.00
38.00
2,937.50
500.00
2,917.00
1,000.00
13,083.33
508.75
3,000.00
29,540.58
$1,023.91
$923.91
100.00 $1,023.91
SEDORE, O'SULLIVAN, LETTERIO, & BARSCHI,
CERTIFIED PUBLIC ACCOUNTANTS, P.C.
PO BOX 918
62 EAST MAIN STREET
WAPPINGERS FALLS, NEW YORK 12590
Tri-Municipal Sewer Comm. 0 & M 2000
PO Box 859
Wappinger Falls, New York 12524-0859
The accompanying Balance Sheet of Tri-Municipa~ Sewer
Commission - General Fund as of October 31, 2000 and the related
Statement o~ Excess (Deficiency) of Revenues Ove~ Expenditures and
Budgetary Statements have been compiled by us in accordance with
standards established by the American Institute of Certified
Public Accountants.
A compilation is limited to presenting in the form of a
financial statement information that is the representation of
management. We have not audited or reviewed the accompanying
financial statements and, accordingly, do not express an opinion
or any other form of assurance on them.
Management has elected to omit substantially all of the
disclosures, and statement of cash flows required by generally
accepted accounting principles. If the omitted disclosures were
included in the financial statements, they might influence the
user's conclusions about the commission's financial position.
Accordingly, these financial statements are not designed for those
who are not informed about such matters.
SEDORE, O'SULLIVAN, LETTERIO, & BARSCHI, CPA's,P.C.
Wappingers Falls, NY
November 1, 2000
TRI-MUNICIPAL SEWER COMMISSION
Operation and Maintenance Fund
Balance Sheet
October 31, 2000
Assets
Petty Cash
Cash - General Checking
Cash - Payroll
Cash - Money Market
Certificate of Deposit
Rents Rec.- Town Poughkeepsie
Rents Rec.- ViI. Wapp. Fls.
Rents Rec.- Town Wappinger
Total Assets
Liablilities and Fund Balance
Liabilities:
Accounts Payable
S.S. & F.W.T. Payable
S.W.T. Payable
D.B.L. Payable
Security Deposit payable- TOW
Total Liabilities
Fund Balance:
Fund Balance - TOWN OF POK
Fund Balance - VILL OF WAPP
Fund Balance - TOWN OF WAPP
Current Year Operations
Total Fund Balance
Total Liabilities and Fund Balance
$
500.00
6,806.10
557.52
228,154.74
250,000.00
13,226.00
32,032.00
29,527.00
$
560,803.36
$ 58,801.17
259.30
23.76
18.00
91. 678.00
150,780.23
120,364.00
264,122.00
32,824.00
(7,286.87)
410,023.13
$ 560,803.36
Unaudited - See Comments in Letter of Transmittal
Tri-MUnicipal Sewer Coma. 0 & M 2000
Operation and Maintenance Fund
Statement of Bxcess (Deficiency) of Revenues Over
Expenditures and BUdgetary Comparisons
10 Months Ended October 31, 2000
Actual For Budgeted For Variance Total
10 Months Ended 10 Months Ended Over/(Under) Annual Budget
Oct. 31. 2000 Oct. 31. 2000 Budget Budget Remaining
Revenues:
Sewer Rents - Twn Poughkeepsi $ 119,379.00 $ 140,595.00 $ (21,216.00) $ 168,714.00 $ 49,335.00
- Vi l. \Iapp. Fls. 320,414.00 316,712.50 3,701.50 380,055.00 59,641.00
Sewer Rents - Twn of \Iapp. 308,054.00 290,538.30 17,515.70 348,646.00 40,592.00
State Grant Income 4,387.00 0.00 4,387.00 0.00 (4,387.00)
Metered \later Revenue 1,251.80 0.00 1,251.80 0.00 (1,251.80)
Interest Income 3,705.39 0.00 3,705.39 0.00 (3,705.39)
Miscellaneous Other Income 9,000.00 0.00 9,000.00 0.00 (9,000.00)
Transfer from Capital 17.000.00 14.166.70 2.833.30 17.000.00 0.00
Total Revenues 783,191.19 762,012.50 21,178.69 914,415.00 131,223.81
Expendi tures :
Administration 0 Personal Ser 12,794.50 12,133.30 661.20 14,560.00 1,765.50
- FICA 1,238.06 825.00 413.06 990.00 (248.06)
o DBl 72.60 83.30 (10.70) 100.00 27.40
o COMPo INS. 0.00 208.30 (208.30) 250.00 250.00
o Equipment 1,882.56 2,500.00 (617.44) 3,000.00 1,117.44
- Office Supp. 3,007.21 2,166.70 840.51 2,600.00 (407.21 )
o Postage 74.00 166.70 (92.70) 200.00 126.00
- InsuranceE&O 5,135.00 4,416.70 718.30 5,300.00 165.00
o Miscelaneous 807.72 833.30 (25.58) 1,000.00 192.28
Contract Servo o legal 11,205.75 8,333.30 2,872.45 10,000.00 ( 1 , 205 .75 )
o Engineer 1,184.00 4,166.70 (2,982.70) 5,000.00 3,816.00
o Administratn 37,263.57 20,000.00 17,263.57 24,000.00 (13,263.57)
o Accountng 28,174.43 35,000.00 (6,825.57) 42,000.00 13,825.57
o Operations 321,810.00 321,805.80 4.20 386,167.00 64,357.00
Equipment - Rental 545.08 333.30 211. 78 400.00 (145.08)
- Replacement 9,520.24 8,333.30 1,186.94 10,000.00 479.76
- Rehab 5yr pl 92,831.75 58,333.30 34,498.45 70,000.00 (22,831.75)
o Repairs 14,912.53 12,500.00 2,412.53 15,000.00 87.47
- Prevo Maint. 6,439.71 5,000.00 1 , 439.71 6,000.00 (439.71)
Plant o Road Care 900.00 3,333.30 (2,433.30) 4,000.00 3,100.00
o Maint.Blg&Gr 5,391.33 5,000.00 391.33 6,000.00 608.67
- Sanitation 1,890.60 2,500.00 (609.40) 3,000.00 1,109.40
o Insurance 15,314.08 15,000.00 314.08 18,000.00 2,685.92
- Chemical Sup 18,695.39 12,500.00 6,195.39 15,000.00 (3,695.39)
o lab.Supply 724.95 2,166.70 (1,441.75) 2,600.00 1,875.05
o lab.Services 11,147.00 9,166.70 1,980.30 11,000.00 (147.00)
Utilities - Electric 146,407.09 155,833.30 (9,426.21) 187,000.00 40,592.91
o Fuel 2,328.71 3,750.00 (1,421.29) 4,500.00 2,171.29
o Telephone. 5,970.83 5,000.00 970.83 6,000.00 29.17
o Water 4,241.32 3,333.30 908.02 4,000.00 (241.32)
Compost o Suppl i es 15,032.05 11,666.70 3,365.35 14,000.00 (1,032.05)
o Disposal 0.00 833.30 (833.30) 1,000.00 1,000.00
- Vehicle R&M 247.81 3,333.30 (3,085.49) 4,000.00 3,752.19
- Vehicle Fuel 3,012.25 1,666.70 1,345.55 2,000.00 (1,012.25)
- licensePermt 7,500.00 6,516.70 983.30 7,820.00 320.00
Contingency 2.775.94 23.273.30 (20.497.36) 27.928.00 25.152.06
Total Expenditures 790.478.06 762.012.30 28.465.76 914.415.00 123.936.94
Bxcess (Deficiency) of
Revenues Over Expenditures $ <7.286.87) $ 0.20 $ (7.287.07) $ 0.00 $ 7.286.87
Unaudited 0 See Comments in letter of Transmittal
Tri-Municipal Sewer Comm. 0 & M 2000
Operation and Maintenance Fund
Statement of Excess (Deficiency) of
Revenues Over Expensitures
10 Months Ended October 31, 2000
1 Month Ended
October 31. 2000
Revenues:
Sewer Rents - Twn Poughkeeps$
- ViI. Wapp. Fls
Sewer Rents - Twn of Wapp.
Total Sewer Rents
13,226.00
32,032.00
29,527.00
74,785.00
State Grant Income
Metered Water Revenue
Interest Income
Miscellaneous Other Income
Transfer from Capital
0.00
0.00
0.00
1,000.00
0.00
Total Revenues
10 Months Ended
Pct October 31, 2000
(17.45)$
(42.27)
(38.96)
(98.68)
0.00
0.00
0.00
(1.32)
0.00
75,785.00 (100.00)
Expenditures:
Administration - Personal Se
- FICA
- DBL
- Equipment
- Office Supp
- Postage
- InsuranceE&
- Miscelaneou
Contract Servo - Legal
- Engineer
- Administrat
- Accountng
- Operations
Equipment - Rental
- Replacement
- Rehab 5yr p
- Repairs
- Prevo Maint
Plant - Road Care
- Maint.Blg&G
- Sanitation
- Insurance
- Chemical Su
- Lab.Supply
- Lab.Service
Utilities - Electric
- Fuel
- Telephone
- Water
Compost - Supplies
- Vehicle R&M
- Vehicle Fue
- LicensePerm
1,169.00
89.43
0.00
539.47
210.46
0.00
0.00
43.88
462.50
0.00
4,139.48
2,304.55
32,181.00
0.00
2,449.50
0.00
1,468.99
0.00
0.00
0.00
0.00
0.00
4,012.63
0.00
2,158.00
10,853.62
0.00
517.63
0.00
0.00
103.65
247.86
0.00
0.00
Contingency
(1. 54)
(0.12 )
0.00
(0.71)
(0.28)
0.00
0.00
(0.06)
(0.61)
0.00
(5.46)
(3.04 )
(42.46)
0.00
(3.23 )
0.00
(1. 94)
0.00
0.00
0.00
0.00
0.00
(5.29 )
0.00
(2.85 )
(14.32)
0.00
(0.68)
0.00
0.00
(0.14)
(0.33)
0.00
0.00
119,379.00
320,414.00
308,054.00
747,847.00
4,387.00
1,251.80
3,705.39
9,000.00
17,000.00
Pct
(15.24)
(40.91)
(39.33)
(95.49)
(0.56 )
(0.16)
(0.47)
(1. 15)
( 2.17)
783,191.19 (100.00)
12,794.50
1,238.06
72.60
1,882.56
3,007.21
74.00
5,135.00
807.72
11,205.75
1,184.00
37,263.57
28,174.43
321,810.00
545.08
9,520.24
92,831.75
14,912.53
6,439.71
900.00
5,391.33
1,890.60
15,314.08
18,695.39
724.95
11,147.00
146,407.09
2,328.71
5,970.83
4,241.32
15,032.05
247.81
3,012.25
7,500.00
2,775.94
Unaudited - See Comments in Letter of Transmittal
(l.63)
(0.16)
(0.01)
(0.24)
(0.38)
(0.01)
(0.66 )
(0.10)
(1. 43)
(0.15 )
(4.76)
(3.60 )
(41.09)
(0.07)
(1.22)
(11.85)
(1. 90)
(0.82 )
(0.11)
(0.69 )
(0.24)
(1.96 )
(2.39 )
(0.09 )
(1. 42)
(18.69)
(0.30 )
(0.76)
(0.54)
(1. 92)
(0.03)
(0.38)
(0.96)
( 0.35)
Tri-Municipal Sewer Comm. 0 & M 2000
Operation and Maintenance Fund
Statement of Excess (Deficiency) of
Revenues Over Expensitures
10 Months Ended October 31, 2000
1 Month Ended
October 31. 2000
10 Months Ended
Pct October 31. 2000
Pct
Total Expenditures
62.951.65 (83.07)
790.478.06 (100.93)
Excess (Deficiency) of
Revenues Over Expenditur$
12.833.35 (16.93)$
(7,286.87)
0.93
Unaudited - See Comments in Letter of Transmittal
TRI-MUNTCTPAL SEWER COMMISSION
QISBURSEMRNT DETAIL
NOVEMBER 2r 2000
TOWN OF WAPPINGER EXPANSION _ 3A
VOUCHER
NUMBER
DATE
VENDOR'S NAME
CHK #
AMOUNT
BILLED
LESS AMOUNT
RETAINAGE OF CHECK
3A-17 10/19/00 Quartararo & Quartararo 2363 $ 5,556.00 -0- $ 5,556.00
3A-17A 10/19/00 Quartararo & Quartararo 2364 165.50 -0- 165.50
3A-17B 10/19/00 Quartararo & Quartararo 2365 1,650.00 -0- 1,650.00
3A-18 10/04/00 M.D. Rudikoff Assoc. 2366 500.00 -0- 500.00
13A-17 11/02/00 Sedore & Company, CPA's 2367 2,917.00 -0- 2,917.00
3A-17 10/31/00 Tri-Municipal 0 & K 2368 1,000.00 -0- 1,000.00
3A-15 10/11/00 Eustance & Horowitz 2369 13,083.33 -0- 13,083.33
I $24,871.83
1$24,871.83 I
Tri-Municipal Sewer Commission
P.O. Box 859
Wappingers Falls, NY 12590
\AU}fER l.lST.II\G
. 11/02/00
J.L Name Voucher" # Inv Date Due Oa"te Inv Amount DiscDate Discount Cash Required
\TT A T & T 6519 10/31/00 11/02/00 61. 00 0 0.00 61.00
3ELl Verizon 6521 10/31/00 11/02/00 251. 95 0 0.00 251. 95
" 6522 10/31/00 11/02/00 63.72 0 0.00 63.72
" 6523 10/31/00 11/02/00 60.95 0 0.00 60.95
BaTT I Bottini Fuel 6524 10/31/00 11/02/00 247.86 0 0.00 247.86
CASH Cash 6525 10/31/00 11/02/00 166.35 0 0.00 166.35
CEllO Cellular One 6526 10/31/00 11/02/00 67.20 0 0.00 67.20
CENHU Central Hudson 6527 10/31/00 11/02/00 6,781.84 0 0.00 6,781.84
" 6528 10/31/00 11/02/00 8n.88 0 0.00 8n.88
DONNE Jacquel ine Donnellan 6529 10/31/00 11/02/00 43.88 0 0.00 43.88
FILTE Fi lter Belts Inc. 6530 10/31/00 11/02/00 2,449.50 0 0.00 2,449.50
GAllA GALLAGHER TRUCK CENTER INC. 6531 10/31/00 11/02/00 103.65 0 0.00 103.65
HOME Home Depot/GECF 6532 10/31/00 11/02/00 73.36 0 0.00 73.36
JDJOH J' D JOHNSON CO. 6533 10/31/00 11/02/00 468.22 0 0.00 468.22
JONES Jones Chemicals Inc 6534 10/31/00 11/02/00 2,565.13 0 0.00 2,565.13
LOWES Lowes 6535 10/31/00 11/02/00 781.09 0 0.00 781. 09
LUCEN Avaya Inc. 6520 10/31/00 11/02/00 34.87 0 0.00 34.87
MIllE LOUIS MIllER PLUMBING, HTG & CONSULT 6536 10/31/00 11/02/00 4,139.48 0 0.00 4,139.48
MMB HSSC 6557 10/31/00 11/02/00 279.79 0 0.00 279.79
NANOS N & S Supply, Inc. 6538 10/31/00 11/02/00 146.32 0 0.00 146.32
OFFIC Office Max 6539 10/31/00 11/02/00 26.97 0 0.00 26.97
POLlU Pollu-Tech Inc. 6540 10/31/00 11/02/00 1,447.50 0 0.00 1,447.50
QUART Quartararo & Quartararo 6541 10/31/00 11/02/00 462.50 0 0.00 462.50
SEDOR Sedore, O'Sullivan Letterio & Barsci 6542 10/31/00 11/02/00 2,304.55 0 0.00 2,304.55
SMITH SMITH ENVIRONMENTAL LABORATORY, INC. 6543 10/31/00 11/02/00 2,158.00 0 0.00 2,158.00
ST Severn Trent Environmental Services 6544 10/31/00 11/02/00 32,181.00 0 0.00 32,181.00
STAPL Staples Credit Plan 6545 10/31/00 11/02/00 17.14 0 0.00 17.14
USMAT U S MATERIALS HANDLING CORP 6546 10/31/00 11/02/00 539.47 0 0.00 539.47
PAEPAIDS :
J. Dorrellan 6516 lO{11/oo 478.80
Ce-rtral Htrlson 6517 10{2O{00 3193.00
J. lJor"mllan 6518 1O{25{00 473.65
lUTAL: $62947.55
.
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~ PRI-OOI
~
AER-OOl
Aerator I A
PRI-002
Aerator I A Aerator 2A
AER -002
Aerator 18
Aerator 18
Aerator 28
Process/Garage
I
Admin. Bldg.
I
~1l/~/oo
$
Headworks Bldg.
Sam p Ie Fungi Bacteria (R2A) Bacteria (T SA)
(CFU/ml) (CFU/ml) (CFU/ml)
PRI-OO 1 > 7,067 424 300
PRI-002 > 7,067 194 212
PRI-003 2,827 4,205 3,922
PRI-003
Aerator 2A
Sam pie Fungi Bacteria (R2A) Bacteria (T SA)
(CFU/ml) (CFU/ml) (CFU/ml)
A ER-OOI 4,6] I 106 548
AER.002 2,192 6,025 5,159
AER.003 > 7,067 3,392 2,703
Aerator 28
AER-003
Sam pie Fungi Bacteria (R2A) Bacteria (TSA)
(CFU/ml) (CFU/ml) (CFU/ml)
SEC-OO 1 2,580 247 106
SEC -002 1,024 177 106
Legend:
e Sample location
Note: Figure not to scale
Figure I - Outdoor Air Sample Locations I
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Laboratory
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PROC
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Entrance
ef "-l
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Conference Room
Process/Garage
Administration Building
,. Fungi Bacteria (R2A) Bacteria (TSA)
Samp Ie (CFU/m3) (CFU/m3) (CFU/m3)
CONFRM 265 Not analyzed 88
LAB 247 Not analyzed 477
PROC 1,166 1,979 3,216
Legend:
. Sample location
Note: Figure not to scale
Figure 2 - Indoor Air Sample Locations
..
,-
$~
Main Entrance
BACK. C
~
Approx. II2 mile
~
00
000
00
COM-002
Compost Facility
Road to
facility exit
COM-OOl 0 0 0 0
00
o 0000
Open-Ended Garage
Sample Fungi Bacteria (R2A) Bacteria (TSA)
(CFU/m3) (CFU/m3) (CFU/m3)
BACK 1,678 71 53
COM-OOl 1,396 671 1,537
CO M -002 1,254 6,431 > 7,067
Legend:
. Sample location
o Compost pile
Note: Figure not to scale
Figure 3 - Compost Facility Sample Locations
~1-\OO
~
MALCOLM PIRNIE, INC.
INDEPENDENT ENVIRONMENTAL ENGINEERS. SCIENTISTS & CONSULTANTS
October 18, 2000
Inder Kumar, Ph.D., P.E., Chief Executive Officer
Eustance & Horowitz, P.C.
P.O. Box 42
Circ1eville, NY 10919
RE: Bioaerosol sampling at Tri-Municipal Sewer Commission
4101.002
Dear Dr. Kumar:
Attached, please find our summary of the bioaerosol sampling at the Tri-Municipal
Sewer Commission from Wednesday September 20,2000.
Very truly yours
1J1~ I ,1 V/1d
VV~X \--.~_~
Mark McGowan, CIH, CSP, Associate
Mirhael Chung, Project Engineer
Malcolm Pirnie, Inc.
104 CORPORATE PARK DR.
BOX 751 WHITE PLAINS, NY 1060J..0751 914-694-2100 914-694-9286 fax http://www.pirnie.com
RECYCLED PAPER
Introduction
On Thursday, September 20, 2000, air samples were taken at the Tri-Municipal Sewer
Commission Waste Water Treatment Pacility (hereafter, referred to as "the facility").
Samples were taken at specific locations throughout the facility to characterize the
microbiological organisms that workers and visitors may encounter while at the facility.
Conditions
Sampling conditions at the facility were excellent. The sky was sunny and clear, with no
clouds. The temperature was in the upper 70s/lower 80s (OP), with a very light breeze
from the southeast. Since it had rained the previous day, conditions favored ample
biological activity, particularly for fungal growth.
As found on The Weather Channel's internet site (htto:llwww.weather.com). the weather
conditions at 3 :20 pm for Poughkeepsie, NY were as follows:
Temperature: 820P
Heat Index: 850P
Wind: Prom southwest @ 10 mph (throughout the sampling, there was very little wind)
Dewpoint: 660P
Relative Humidity: 58%
Barometer: 29.82", falling
Visibility: 10 miles
Means and Methods
Samples were taken throughout the facility using an Andersen N6 sampler, and a pump
calibrated to draw 1 ft3/minute (28.3 L1min) through the sampler. Sample collection
times were 2 minutes. Samples were collected using sterile technique on three agar
plates per location. Three types of agar plates were used to collect samples:
· Malt extract agar (MEA) - environmental mold/fungi analysis
· R2A medium - water bacteria analysis
· Tryptic soy agar (TSA) - environmental bacteria analysis
At each sample location, the Andersen N6 sampling unit was sterilized with an alcohol
prep. At each location, ambient air samples were collected for 2 minutes onto each plate.
Two of the R2A plates were unusable due to laboratory contamination. The typical
indoor air quality investigative protocol calls for two plates (MEA and TSA) and this
protocol was adhered to for the samples in the Administration Building. The Andersen
N6 sampling unit design resembles an impactor unit; during sampling, air impacts the
media in 400 discrete points. After sampling, each plate was closed, sealed, and shipped
overnight to P&K Laboratories, Cherry Hill, NJ, for culturing and analysis.
2
Samples were taken throughout the facility as summarized in Table 1.
Table 1 - Sample Locations
Location Samples Sample Number(s) Plates Used
Primary Clarifiers 3 PRl-OOI, PRl-002, MEA, R2A, TSA
PRl-003
Aeration Tanks 3 AER-OOI, AER-002, MEA, R2A, TSA
AER-003 -,
Secondary Clarifiers 2 SEC-OO I, SEC-002 MEA, R2A, TSA
Process Garage I PROC MEA, R2A, TSA
Administration Building 2 CONF RM, LAB MEA, TSA
Compost Facility 2 COM-OOI, COM-002 MEA, R2A, TSA
Control Sample I BACK MEA, R2A, TSA
Figure 1 illustrates the sampling locations for the primary clarifiers, aeration tanks, and
secondary clarifiers. Sample locations in the vicinity of the wastewater treatment plant
operations were chosen to represent upwind, central, and downwind evaluation points.
At the time of sampling, the wind blew from the southeast and was deflected to the
northwest by the river bluff east of the plant. For the primary clarifiers, PRI-OOI
represents the upwind sampling location, PRI -002 represents the central sampling
location, and PRI-003 represents the downwind sampling location, but this is at the edge
of Aeration Tank 2A which was active during the entire sampling event.
For the aeration tanks, AER-OOI represents the upwind sampling location, AER-002
represents the central sampling location, and AER-003 represents the downwind
sampling location. Sample AER-OOI was taken while the aeration tanks remained turned
off (these units had been off prior to arriving at the facility), while samples AER-002 and
AER-003 were taken while the aeration tanks were turned on.
For the secondary clarifiers, SEC-OOI represents the upwind sampling location, and SEC-
002 represents the downwind sampling location. At each sampling location, all three
types of plates were used to characterize the quantities and types of fungi, waterborne
bacteria, and airborne bacteria found in the immediate environment.
Figure 2 illustrates the sampling locations for the administration building and process
garage. One sample was taken in the conference ro~m; the sample was taken from atop
the conference table, with the doorway to the office open. One sample was taken in the
laboratory; the sample was taken from atop the bench in front of the window facing the
waste water treatment plant operations, with the doorway to the lobby area open, and the
doorway to the process garage closed. These samples were taken to be representative of
the microbiological air quality within the administration building. The MEA and TSA
plates are sufficient to characterize fungal and bacterial organisms in indoor air.
One sample was taken in the process garage; the sample was taken in the middle of the
garage, atop a potassium permanganate tank (approximately 5-Y4 ft. above the ground), in
front of the belt filter press. All three plates were used in this location to characterize the
3
quantities and types of fungi, waterborne bacteria, and airborne bacteria that are found in
the immediate environment.
Figure 3 illustrates the sampling locations for the compost facility and the control sample.
Sample locations in the vicinity of the compost facility were chosen to represent upwind
and downwind exposure points. At the time of sampling, the wind blew from the west
(toward the east). As such, COM-OOI represents the upwind sampling location, and
COM-002 represents the downwind sampling location.
The background sample was taken near the fenced pump station at the maIn entrance to
the facility. This sample was taken to represent quantities and types of fungi, waterborne
bacteria, and airborne bacteria that could be considered as "backgroLL.""ld" levels for the
environment.
Results
The results from the sampling are presented in Table 2. Results are presented by sample,
by media used, by organism detected, and finally, in the number of viable organisms
detected expressed in colony forming units per cubic meter (CFU/m\ The total numbers
of viable organisms for each medium and each sampling location are presented on
Figures 1,2 and 3.
As described earlier, air was drawn through the sampling unit for 2 minutes at a rate of 1
ft3/min (28.3 L/min). After culturing, the number of colonies per plate was counted, and
the number of colonies per plate was converted to a number of colony forming units per
cubic meter of ambient air. If more than 400 colonies grew on a plate (i.e., more colonies
them spaces available on the plate), then the plate was considered "overloaded". In such a
case, microorganisms can be identified, and the dominant organism can be identified, but
exact quantities of each microorganism can not be quantified.
Outdoor Air Samples
The results of the environmental mold/fungi samples in the area of the primary clarifiers,
aerators, secondary clarifiers, and sludge holding tanks indicate that mold/fungal levels
tend to be higher at the upwind locations. These results are summarized in Table 3.
Table 3 - Environmental Mold/Fungi Sample Results
by Relative Wind Location
Location Upwind (CFU/m') Central (CFU/mj) Downwind (CFU/m ')
Primary Clarifiers >7,067 >7,067 2,827
Aeration Tanks 4,611 2,192 >7,067
Secondary Clarifiers 2,580 - 1,024
Background 1,678
4
As compared to the background sample, mold/fungal levels at these locations are
generally higher than background mold/fungal levels (within the same order of
magnitude). On the day of sampling, the results generally seem to indicate that the
airborne mold/fungal organism/spore concentrations around the facility's wastewater
treatment plant operations are more attributable to environmental background than to the
sewage waste being treated. Due to the previous day's rain event and consequent
moisture levels, relatively high airborne concentrations of mold and fungi were expected.
The results of the bacterial samples in the areas of the primary clarifiers and aeration
tanks indicate that airborne bacterial concentrations tend to be higher at "the downwind
side of these units. These results are summarized in Table 4.
Table 4 - Environmental Bacteria Sample Results
by Relative Wind Location
Location Upwind (CFUlm') Central (CFUlm') Downwind (CFUlmj)
Primary Clarifiers 424/300 194/212 4,205/3,922
Aeration Tanks 106/548 6,025/5,159 3,392/2,703
Secondary Clarifiers 247/106 . 177/106
Background 71/53
Note: Waterborne bacteria are listed first, followed by airborne bacteria.
The values for the primary clarifiers trend downward when moving from the upwind
sampling location to the central sampling location, but the last, downwind values were up
by a factor of about 20. It is believed that this last value was strongly influenced by
emissions from Aeration Tank 2A.
Airborne concentrations of bacteria in the upwind air of the plant were comparable to the
concentrations detected in the background sample.
As compared to the background and upwind samples, bacterial concentrations at the
downwind primary clarifier location and the central and downwind aerator locations are
consistently higher.
As, described previously, the central and downwind samples in the area of the aerators
were taken after restoring power to a long unused portion of the system. As such, higher
levels of bacteria (waterborne) in the downwind air are most likely attributable to the
wastewater processing in the aeration tanks.
Indoor Air Samples
The results of the environmental mold/fungi sample in the conference room indicate that
airborne mold/fungal concentrations were well below background airborne mold/fungal
concentrations. The airborne environmental bacteria concentrations in the conference
room were below levels detected in the plant and equivalent to background
environmental bacteria levels. These results are summarized in Table 5.
5
Table 5 - Environmental Sample Results in Indoor Air
Compared to Outdoor Background Levels
Environmental Environmp.ntal
Location Mold/Fungi (CFU/mJ) Bacteria (CFU/mJ)
Conference Room 265 88 (Airborne)
Laboratory 247 477 (Airborne)
Process/Garage 1,166 1,979/3,216
Background 1,678 71/53
Note: Waterborne bacteria are listed first, followed by airborne bacteria.
The results of the environmental mold/fungi sample in the laboratory indicate that
airborne mold/fungal concentrations were equivalent to the levels detected in the
conference room, and below background airborne mold/fungal concentrations. The
environmental bacteria concentrations in the laboratory were roughly equivalent to the
upwind concentrations at the plant but above the concentrations detected in the
conference room and background samples.
The results of the environmental mold/fungi sample in the process garage indicate that
airborne mold/fungal concentrations were comparable to background mold/fungal levels.
The environmental and waterborne bacterial airborne concentrations in the process
garage were comparable to those detected near the downwind side of the aerators, and
higher than background airborne bacterial levels.
Compost Facility Samples
The results of the environmental mold/fungi samples at the compost facility indicate that,
on the day of sampling, the mold/fungal concentrations detected were comparable to
background concentrations and that the facility had no apparent effect on the downwind
airborne mold/fungal concentrations. These results are summarized in Table 6.
Table 6 - Environmental Sample Results at the Compost Facility
Compared to Outdoor Background Levels
Environmental Environmental
Location Mold/Fungi (CFU/mJ) Bacteria (CFU/mJ)
Compost, upwind 1,396 671/1,537
Compost, downwind 1,254 6,431/ >7,067
Background 1,678 71/53
Note: Waterborne bacteria are listed first, followed by airborne bacteria.
The results of the environmental bacterial samples in the compost facility indicate that
the airborne concentrations found at the downwind sampling location were higher by a
factor of 10 than the levels found at the upwind sampling location. Environmental
6
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bacteria concentrations at both locations of the compost facility were found to be higher
than background airborne bacterial levels.
Discussion
From the results, it appears that much of the outdoor mold/fungal concentrations are
attributable to ambient environmental conditions. Outdoor bacterial concentrations
appear to be affected by the operation of the wastewater treatment plant equipment,
particularly equipment that sprays or splashes water or moves composted'sludge. As for
bioaerosol conditions, the conference room air quality is better than ambient conditions.
The laboratory air quality is comparable to ambient conditions. This may be the result of
a greater number of air changes per hour designed into a laboratory HV AC system.
See Table 6 above for a summary of viable toxic and/or pathogenic fungi concentrations
by sample. The fungi that were identified in each outside sampling location are
representative of common saprophytic environmental fungi, with Basidiomycetes and
Cladosporium as the most common genera. Aspergillus, Cladosporium and Mucor are
opportunitistic pathogens. Aspergillus, Penicillium, Fusarium and Alternaria are
mycotoxic.
The bacteria that were identified in each outside sampling location are representative of
common environmental bacteria with various gram-negative bacteria the predominant
group of organisms detected. Other common genera detected were Bacillus,
Pseudomonas sp. non aeruginosa, Stenotrophomonas maltophilia, Rhodococcus, and
Methylobacterium. Staphylococcus, a human cohabitant bacteria, was detected around
the aerators and in the upwind Compost Facility air sample.
8
ASPERGILLUS
There are more than 160 different species of Aspergillus, 16 of which have
been documented as etiological agents of human disease. The aspergilli
are probably the most common group of fungi in our environment. Many
species of the genus are frequently isolated from a variety of substrata,
including forage products, grains, nuts, cotton, organic debris and water
damaged organic building materials.
Because of the ubiquity of the aspergilli within the environment, man is
constantly exposed to these fungi. The diseases caused by species of
Aspergillus are relatively uncommon and are rarely found in individuals with
normally functioning immune systems. However, due to the substantial
increase in populations of individuals with active immune suppression, such
as individuals with HIV, chemotherapy patients and those on corticosteriod
treatment, contamination of building substrates with fungi, particularly
Aspergillus species have become an increasing concern. Aspergillosis is now
the second most common fungal infection requiring hospitalization in the
United States.
The most frequently encountered opportunistic Aspergillus pathogen,
Aspergillus fumigatus, is seen most abundantly in decomposing organic
materials. Because it grows well at temperatures up to 55iC, self-heating
compost piles provide an excellent environment for the fungi. Compost
made up of chipped branches and leaves will often yield a massive and
virtually pure culture of A. fumigatus. A. fumigatus has been reported to be
the major organism isolated from air samples obtained near compost sites.
People who handle compost or decomposing haystacks often develop
hypersensitivity to spores of Aspergillus and after exposure may suffer a
severe allergic response.
Aspergillus flavus is the second most frequently encountered fungi in cases
of infection with Aspergillus species. In addition to causing infections,
Aspergillus flavus is also renowned for its production of aflatoxin, one of
the most potent carcinogens known to man. Concern about aflatoxin began
in the 1960s after some 100,000-turkey poults in Great Britain died as a
result of ingesting feed tainted with aflatoxin. When it became evident that
aflatoxin was highly carcinogenic most industrialized countries established
tolerances for aflatoxin levels in food and feeds. The risks associated with
airborne exposure to aflatoxin in contaminated buildings, as with other
mycotoxins, has not been adequately studied.
Aspergillus niger is the third most common aspergilli associated with
disease and is more common than any other Aspergillus species within the
genus and is found in and upon the greatest variety of substrates. It is
commonly associated with "fungus ball", a condition wherein fungus
actively grows in the human lung, fonning a ball, without invading lung
tissue.
Because invasive aspergillosis occurs most frequently among highly
immunosuppressed patients, the presence of Aspergillus spores in hospital
air has important implications. Aspergillus spores frequently occur in
hospitals throughout the world and a number of severe outbreaks resulting
in deaths due to disseminated invasive aspergillosis have been reported
following renovation activities in hospitals. A study of 39 bone marrow
transplant patients who resided in rooms equipped with whole wall laminar
flow HEP A filtration units reported no cases of nosocomial aspergillosis. In
sharp contrast, 14 cases of nosocomial aspergillosis in 74 bone marrow
transplant recipients occurred in-patients housed elsewhere. It is critical
that adequate engineering controls are implemented during renovations at
hospitals or at any facility the immunosuppressed frequent.
Alternaria sp.:
Alternaria is a very common fungal saprophyte found on decaying wood, compost,
plants, food, and in different types of soil. Its distribution is worldwide and
it is frequently found in outdoor air, where in temperate climates the conidia
(spore) levels reach their peak in late summer. Indoors it can be found in
house dust, in carpets, on damp spots around showers and around window frames
and any area where condensation occurs.
Because of its abundance and ubiquity, Alternaria is one of the most important
fungal allergens. Allergy to Alternaria will most often cause an" immediate IgE
mediated allergic response, however, there have been documented cases of
hypersensitivity pneumonitis (IgG mediated) type reactions to airborne exposure
of Alternaria spores. Infection with Alternaria is extremely rare.
When grown on malt extract agar at 25 degrees Celsius, the colonies reach a
diameter of 6 cm in 7 days. Colonies are typically black to olivaceous-black or
gray. Macroscopically, it appears similar to molds in the genera Stemphylium,
Ulocladium and Curvularia; however, their microscopic morphology is quite
different. Alternaria conidia are very characteristic, being ovoid to pear
shaped and forming in chains.
Mycotoxins produced by Alternaria have not been subject to much investigation.
It is known that Alternaria alternata, the most common Alternaria species,
produces alternariol, an antifungal metabolite. Other specific metabolites
include altertoxin I and II, alternariol monomethylether, altenuene, altenusin
and tenuazonic acid.
Health Effects of Fungi:
Fungi occur in nature and as such are always present in air at ambient levels. Although these levels
vary considerably, depending on the locale and current prevailing environmental conditions, most
individuals won't suffer adverse health effects except possibly allergic responses at these levels.
However, in buildings, conditions can be favorable for fungal growth (e.g. moisture problems)
resulting in increasing the levels of airborne fungal spores by several orders of magnitude.
Inhalation of such elevated levels of airborne spores can result in allergenic or toxic responses.
Although infection can even occur in an otherwise healthy individual occasional!y, high levels of
fungi in indoor environments are of particular concern with infants, elderly, and immune
compromised individuals who are much more susceptible to fungal infections. Health effects from
exposure to fungi can be divided into four general categories: infection, toxicosis, allergy and
irritation.
Infection
Many fungi are purely saprophytic, using dead or decaying organic matter for food. However, there
are now over 100 species that are known to cause infection in humans. There are three
classifications of infection caused by fungi: systemic, opportunistic and dermatophytic.
· Systemic Infection: Systemic fungal infections include: Histoplasmosis, ,Coccidioidomycosis,
Blastomycosis and Paracocidioidomycosis. In most cases infection is initiated when spores are
of the fungi that cause these diseases are inhaled. A large majority ofthese infections are self-
limiting and produce minimal or no symptoms. However immune suppressed individuals may
develop chronic localized infections or the disease may disseminate throughout the body, which
generally proves fatal.
· Opportunistic Infection: Opportunistic infections are generally limited to individuals with
impaired immunological defenses, where infection is secondary to a primary disease or
condition. The opportunistic fungi are facultative parasites, meaning they can use both living
and dead substrates for nutrients. Common opportunistic fungi include species of Aspergillus,
Candida, Cladosporium, Mucor, Rhizopus and Cryptococcus.
· Dermatophytes: Dermatophytes are a group of fungi that infect the hair, skin and nails.
Infection usually occurs through direct contact with an infected individual or indirectly by
sharing clothes, grooming utensils, towels, etc. Transmission to humans from an environmental
source is extremely rare, although outbreaks from soil have been reported.
Toxicosis
Many fungi produce toxic metabolites called mycotoxins. The health effects from exposures to the
levels of mycotoxins that may be encountered in contaminated indoor environments are not yet
completely known. However, dramatic toxic and carcinogenic effects have been recorded for
animals and humans exposed to high levels of mycotoxins in laboratory studies or agricultural
situations. Some of the more commonly known mycotoxins are aflatoxin, sterigmatocystin and
ochratoxin, produced predominantly by Aspergillus and Penicillium species, and T -2 toxin,
vomitoxin, fumonisin, zearalenone, satratoxins and other tricothecene mycotoxins produced by
Fusarium and Stachybotrys species. There are numerous other mycotoxins produced by a fungi, of
which the health effects remain unknown. Generally mycotoxins are nonvolatile and inhalation
exposure usually occurs only after disturbance of a contaminated source. Symptoms of exposure to
mycotoxins may include cold and flu like symptoms, headache, nose bleeds, dermatitis and
immune suppression. Many mycotoxins are highly carcinogenic.
Clarification - Potential Mycotoxins in Indoor Environments
This document provided a summary of certain mycotoxin producing fungi and their associated
health effects. It should be emphasized that these health effects are largely derived from ingestion
and animal field studies, not inhalation and therefore cannot necessarily be extrapolated to indoor
environments. While inhalation experiments have only been performed with a very limited number
of mycotoxins, these studies suggest that toxicity may be greater via inhalation. What is not
presently known is whether indoor exposures to mycotoxins are of sufficient magnitude to cause
the adverse effects seen in these studies. In addition, unlike experimental studies, indoor (and
outdoor) field exposures are to mixtures of molds and their products.
Allergy
Allergy is the most common symptom associated with exposure to elevated levels of fungal spores
or mycelial fragments. Any fungus can be allergenic, producing antigenic proteins and
polysaccharides that can cause allergic reactions in sensitive individuals. Symptoms include
conjunctivitis, rhinitis, bronchitis, asthma and hypersensitivity pneumonitis.
The first step in the allergic process is sensitization, which occurs from an initial exposure to an
antigen. During the sensitization process there may be no symptoms, however, the body's immune
system produces antibodies to the antigen. Following each additional exposure the antibodies react
with the antigen resulting in a release of histamine and other inflammatory response agents. All
people produce antibodies, however, certain people with genetic predisposition to allergy, produce
significantly greater quantities, which continues for years after an encounter with an allergen.
Irritation
Fungi produce volatile organic compounds during degradation of substrates that cause the "moldy"
odor associated with fungal contamination. These compounds can be irritating to mucous
membranes, causing headaches and other symptoms.
TABLE 2
SUMMARY OF AIR SAMPLING RESULTS FROM TRI-MUNICIPAL SEWER COMMISSION
Medium FungallBacterial1D Colony Conc'n
Sample ID Air Vol (L) Counts (CFUlml) (*) Percent (n)
Fungi
PRl-oO I 56.6 MEA OVERLOADED >400 > 7,067 NQ
Basidiomycetes NQ DOMINANT NQ
Cladosporium NQ NQ
Penicillium NQ NQ
.,
Bacteria
56.6 R2A Actinomycetes 2 35 8
Bacillus 4 71 17
Flavobacterium 2 35 8
gram-negative bacteria and others a 12 212 50
Methylobacterium 2 35 8
Staphylococcus 2 35 8
Total: 24 424 99
Bacteria
56.6 TSA Acinetobacter 3 53 18
Bacillus 2 35 12
Flavobacterium I 18 6
gram-negative bacteria and others 4 71 24
Pseudomonas sp. non aeruginosa 2 35 12
Stenolrophomonas maltophilia 5 88 29
Total: 17 300 101
Fungi
PRI-002 56.6 MEA OVERLOADED >400 > 7,067 NQ
Alternaria alternata NQ NQ
Basidiomycetes NQ DOMINANT NQ
Cladosporium NQ NQ
Phoma NQ NQ
Yeasts NQ NQ
Bacteria
56.6 R2A Acinetobacter I 18 9
Flavobacterium 2 35 18
gram-negative bacteria and others 3 53 27
Methylobacterium 2 35 18
Staphylococcus 1 18 9
Stenotrophomonas maltophilia 2 35 18
Total: II 194 99
Bacteria
56.6 TSA Acinetobacter 3 53 25
gram-negative bacteria and others 3 53 25
Methylobacterium 3 53 25
Pseudomonas sp. non aeruginosa I 18 8
Stenotrophomonas maltophilia 2 35 17
Total: 12 212 100
Page I of7
TABLE 2 (continued)
SUMMARY OF AIR SAMPLING RESULTS FROM TRI-MUNICIPAL SEWER COMMISSION
Colony Conc'n
Sample ID Air Vol (L) Medium Fungal/BacteriallD Counts (CFU/ml) (.) Percent (U)
Fungi
PRI-003 56.6 MEA Basidiomycetes 44 777 28
Cladosporium III 1,961 69
Epicoccum nigrum I 18 < I
Nigrospora sphaerica 1 18 " < I
Penicillium I 18 < I
Phoma I 18 < I
Pithomyces chartarum I 18 <I
Total: 160 2,827 100
Bacteria
56.6 R2A Bacillus 9 159 4
Flavobacterium 3 53 I
gram-negative bacteria and others a 147 7,,597 62
Methylobacterium 5 88 2
Pseudomonas sp, non aeruginosa 5 88 2
Staphylococcus 7 124 3
Stenotrophomonas maltophilia 62 1,095 26
Total: 238 4,205 100
Bacteria
56.6 TSA Bacillus 14 247 6
Flavobacterium 3 53 1
gram-negative bacteria and others a 109 1,926 49
Methylobacterium 3 53 I
Pseudomonas sp. non aeruginosa 10 177 5
Stenotrophomonas maltophilia 83 1,466 37
Total: 222 3,922 99
Fungi
AER-OOI 56.6 MEA Alternaria altemata 16 283 6
Cladosporium 216 3,816 83
Curvularia 6 106 2
Epicoccum nigrum 3 53 I
Penicillium 3 53 I
Pithomyces chartarum IS 265 6
Sterile fungi I 18 <I
Trichoderma barzianum I 18 < I
Total: 261 4,611 100
Bacteria
56.6 R2A Bacillus 2 35 33
gram-negative bacteria and others 2 35 33
Staphylococcus 2 35 33
Total: 6 106 99
Bacteria
56.6 TSA Flavobacterium 2 35 6
gram-negative bacteria and others a 12 212 39
Methylobacterium 4 71 13
Pseudomonas sp. non aeruginosa 3 53 10
Rhodococcus 5 88 16
Slenotrophomonas maltophilia 5 88 16
Total: 31 548 100
Page 2 of7
TABLE 2 (continued)
SUMMARY OF AIR SAMPLING RESULTS FROM TRI-MUNICIPAL SEWER COMMISSION
Medium Colony Conc'n
Sample ID Air Vol (L) Fungal/BacteriallD Counts (CFUlml) (0) Percent (00)
Fungi
AER-002 56.6 MEA Alternaria altemata I 18 < I
Basidiomycetes 94 1,661 76
Cladosporium 27 477 22
Curvularia 1 18 < I
Epicoccum nigrum I 18 < 1
Total: 124 2,192 100
Bacteria
R2A Bacillus 8 141 2
Flavobacterium 8 141 2
gram-negative bacteria and others a 64 1,131 19
Pseudomonas sp. non aeruginosa 33 583 10
Staphylococcus 43 760 13
Stenotrophomonas maltophilia 185 3,269 54
Total: 341 6,025 100
Bacteria
56.6 TSA Bacillus 34 601 12
Flavobacterium 2 35 < 1
gram-negative bacteria and others a 65 1,148 22
Pseudomonas sp. non aeruginosa 31 548 11
Shewanella putrefaciens 8 141 3
Staphylococcus 2 35 < 1
Stenotrophomonas maltophilia 150 2,650 51
Total: 292 5,159 100
Fungi
AER-003 56.6 MEA OVERLOADED >400 > 7,067 NQ
Alternaria a1temata NQ NQ
Basidiomycetes NQ DOMINANT NQ
Cladosporium NQ NQ
Penicillium NQ NQ
Yeasts NQ NQ
Bacteria
56.6 R2A Bacillus 4 71 2
Flavobacterium 5 88 3
gram-negative bacteria and others a 43 760 22
Methylobacterium 7 124 4
Pseudomonas sp. non aeruginosa 29 512 15
Staphylococcus 21 371 11
Stenotrophomonas mallophilia 83 1,466 43
Total: 192 3,392 100
Bacteria
56.6 TSA Bacillus 10 177 7
gram-negative bacteria and others a 34 601 22
Methylobacterium 3 53 2
Pseudomonas sp. non aeruginosa 38 671 25
Staphylococcus 12 212 8
Stenotrophomonas maltophil ia 56 989 37
Total: 153 2,703 101
Page 3 of?
TABLE 2 (continued)
SUMMARY OF AIR SAMPLING RESULTS FROM TRI-MUNICIPAL SEWER COMMISSION
Sample ID Air Vol (L) Medium Fungal/BacteriallD Colony Conc'n
Counts (CFUlm3) (*) Percent (U)
Fungi
SEC-OOI 56.6 MEA Alternaria alternata 2 35 I
Basidiomycetes 116 2,049 79
Cladosporium 25 442 17
Penicillium I 18., < I
Phoma I 18 < I
Trichodenna harzianum I 18 < I
Total: 146 2,580 100
Bacteria
56.6 R2A Bacillus I 18 7
Flavobacterium 3 53 21
gram-negative bacteria and others 3 53 21
Staphylococcus 6 106 43
Stenolrophomonas maltophilia I 18 7
Total: 14 247 99
Bacteria
56.6 TSA Actinomycetes I 18 17
Bacillus I 18 17
gram-negative bacteria and others a 2 35 33
Methylobacterium I 18 17
Staphylococcus I 18 17
Total: 6 106 101
Fungi
SEC-002 56.6 MEA Alternaria altemata I 18 2
Basidiomycetes 26 459 45
Cladosporium 24 424 41
Fusarium 2 35 3
Phoma 2 35 3
Sterile fungi I 18 2
Yeasts 2 35 3
Total: 58 1,024 99
Bacteria
56.6 R2A Flavobacterium I 18 10
gram-negative bacteria and others 4 71 40
Methylobacterium 2 35 20
Stenolrophomonas maltophilia 3 53 30
Total: 10 177 100
Bacteria
56.6 TSA Bacillus I 18 17
gram-negative bacteria and others I 18 17
Pseudomonas sp. non aeruginosa 2 35 33
Rhodococcus I 18 17
Shewanella pUlrefaciens I 18 17
Total: 6 106 101
Page 4 of7
TABLE 2 (continued)
SUMMARY OF AIR SAMPLING RESULTS FROM TRI-MUNICIPAL SEWER COMMISSION
Air Vol (L) Medium Colony Conc'n
Sample ID Fungal/Bacterial [0 Counts (CFU/ml) (*) Percent(U)
Fungi
CONFRM 56.6 MEA Basidiomycetes 6 [06 40
Cladosporium 6 106 40
Sterile fungi I [8 7
Tritirachium 2 35 .. 13
Total: 15 265 [00
Bacteria
56.6 TSA Actinomycetes [ [8 20
gram-negative bacteria and others 2 35 40
Micrococcus luteus ( 18 20
Staphylococcus I [8 20
Total: 5 88 100
Fungi
LAB 56.6 MEA Cladosporium 7 [24 50
Fusarium [ 18 7
Penicillium 2 35 [4
Pithomyces chartarum 2 35 14
Tritirachium I 18 7
Yeasts I 18 7
Total: 14 247 99
Bacteria
56.6 TSA Bacillus 6 106 22
Flavobacterium I 18 4
gram-negative bacteria and others 2 35 7
Methylobacterium 4 71 15
Micrococcus (uteus 4 71 15
Pseudomonas sp. non aeruginosa 2 35 7
Rhodococcus 5 88 19
Staphylococcus 3 53 11
Total: 27 477 [00
Page 50f7
TABLE 2 (continued)
SUMMARY OF AIR SAMPLING RESULTS FROM TRI-MUNICIPAL SEWER COMMISSION
Fungal/Bacterial1D Colony Conc'n
Sample ID Air Vol (L) Medium Counts (CFUlm3) (*) Percent (..)
Fungi
PROC 56.6 MEA Basidiomycetes 42 742 64
Cladosporium 21 371 32
Penicillium 2 35 3
Sterile fungi I 18 .. 2
Total: 66 1,166 101
Bacteria
56.6 R2A Bacillus 25 442 22
gram-negative bacteria and others a 30 530 27
Methylobacterium 6 106 5
Pseudomonas sp. non aeruginosa 17 300 IS
Stenotrophomonas maltophil ia 34 601 30
Total: 112 1,979 99
Bacteria
56.6 TSA Actinomycetes 3 53 2
Bacillus 52 919 29
gram-negative bacteria and others a 61 1,078 34
Pseudomonas sp. non aeruginosa 24 424 13
Stenotrophomonas maltophilia 42 742 23
Total: 182 3,216 101
Fungi
COM-oOI 56.6 MEA Aspergillus versicolor 4 71 5
Basidiomycetes 20 353 25
Cladosporium 46 813 58
Mucor 2 35 3
Penicillium 5 88 6
Phoma I 18 I
Sterile fungi I 18 I
Total: 79 1,396 99
Bacteria
56.6 R2A Actinomycetes 10 177 26
Flavobacterium 2 35 5
gram-negative bacteria and others a 17 300 45
Methylobacterium 4 71 II
Rhodococcus I 18 3
Staphylococcus 4 71 II
Total: 38 671 101
Bacteria
56.6 TSA Actinomycetes 12 1,212 83
Bacillus 2 35 2
gram-negative bacteria and others a 9 159 10
Methylobacterium 2 35 2
Pseudomonas sp. non aeruginosa 2 35 2
Total: 87 1,537 99
Page 6 of 7
TABLE 2 (continued)
SUMMARY OF AIR SAMPLING RESULTS FROM TRI-MUNICIPAL SEWER COMMISSION
Medium Fungal/BacteriallD Colony Conc'n
Sample ID Air Vol (L) Counts (CFU/m3) (.) Percent (U)
Fungi
COM-002 56.6 MEA Alternaria alternata I 18 1
Aspergillus fumigatus I 18 1
Basidiomycetes 28 495 39
Chaetomium globosum 1 18 .. 1
Cladosporium 37 654 52
Pithomyces chartarum 3 53 4
Total: 71 1.254 98
Bacteria
56.6 R2A Actinomycetes 342 6,042 94
Bacillus 2 35 < 1
gram-negative bacteria and others a 9 159 2
Methylobacterium 4 71 1
Pseudomonas sp. non aeruginosa 3 53 < 1
Stenotrophomonas maltophilia 4 71 I
Total: 364 6,431 100
Bacteria
56.6 TSA OVERLOADED - >400 > 7,067 NQ
Actinomycetes NQ DOMINANT NQ
Bacillus NQ NQ
gram-negative bacteria and others a NQ NQ
Methylobacteriurn NQ NQ
Pseudomonas sp. non aeruginosa NQ NQ
Stenotrophomonas maltophilia NQ NQ
Fungi
BACK 56.6 MEA Alternaria alternata 2 35 2
Basidiomycetes 62 1,095 65
Cladosporium 27 477 28
Curvularia 1 18 I
Phoma I 18 I
Sterile fungi 1 18 1
Yeasts 1 18 1
Total: 95 1,678 99
Bacteria
56.6 R2A Bacillus I 18 25
gram-negative bacteria and others 3 53 75
Total: 4 71 100
Bacteria
56.6 TSA Flavobacterium 1 18 33
gram-negative bacteria and others 2 35 67
Total: 3 53 100
Notes:
.: Concentration is (CFU/sample volume); the given total may not equal the sum of the given numbers due to rounding.
u: The percentage of each group of fungi or bacteria is for the total population; the sum may not equal 100 due to rounding.
NQ: Not Quantifiable.
Page 70f7